|
|
Detection of Farthest Node in Wireless Sensor Networks
Pfudl, Tomáš ; Morávek, Patrik (referee) ; Šimek, Milan (advisor)
Master’s thesis in the first part focuses on the effectiveness of communication in the selection of reference nodes during the anchor-free localization in WSN. In this part two anchor-free localization algorithms AFL and CRP are explored. Knowledge learned on the selection of reference nodes and communication complexities of these algorithms are used in the second part. The second part is aimed at eliminating weaknesses in communication and subsequently proposed a new algorithm for selection of references. The principle of the newly proposed algorithm CASRU (Centralized Algorithm for Selection of Reference Nodes) is based on the principle of CRP. CASRU algorithm builds logical tree structure and then filters the communication through it. New algorithm achieves much greater success in the selection in randomly distributed networks while reducing the amount of communication load.
|
|
|
Inflammatory Response after the Implantation of Bioresorbable Vascular Scaffolds vs. 2nd Generation Druh-eluting Stents
Novotný, Vojtěch ; Šťásek, Josef (advisor) ; Kala, Petr (referee) ; Kočka, Viktor (referee)
The concept of bioresorbable scaffolds (BVS) originated in the 90s of the 20th century, however, the first commercially available coronary BVS was Absorb BVS in 2011. The dissertation deals with aspects of working with this type of BVS and its use in real practice. We focused on examining the inflammatory response after implantation of Absorb BVS in comparison with the proven 2nd generation metallic drug eluting stent (DES). The introduction systematically discusses the topic of BVS from history, through the composition of BVS and implantation techniques to available literature data. The second topic discussed is the inflammatory response after coronary stent implantation, we provide an overview of available data from studies and the introduction of inflammatory markers, which were analyzed in the main study. The presented work includes three main studies. A Pilot study in which we evaluated the systemic inflammatory response to coronary artery trauma caused by percutaneous coronary intervention previously described in the literature. We verified that the selected laboratory-determined markers of systemic inflammation (hs-CRP, IL-6 and serum amyloid A) increase significantly 24/48 hours after PCI compared to basal. This pilot study was directly followed by the Randomized study, in which we randomly...
|
|
|
Periopathogens and systemic markers in etiology and diagnostics of periodontal and cardiovascular diseases.
Myšák, Jaroslav ; Foltán, René (advisor) ; Izakovičová Hollá, Lydie (referee) ; Markovská, Neda (referee)
Periodontitis is a disease primarily affecting tooth attachment, i.e. concerning oral cavity, however, its connection to a number of systemic diseases is apparent nowadays. Oral microorganisms and their interaction with the immune system of their host play a significant part in the etiology of this multifactorial disease. Rapid development of DNA-based diagnostic methods in the last 15 years dramatically increased the spectrum of identified oral microorganisms and promoted understanding of how particular taxons correlate with periodontal health or disease. Moreover, next generation sequencing methods also bring new possibilities to study the relationship between periodontitis and other diseases such as diabetes mellitus, cardiovascular diseases (CVD), stroke, lung infection or kidney diseases, etc. The presented work focuses on the use of sequencing methods to compare the taxonomic composition of microbiomes within oral cavity and the tissues of aortic valves in patients suffering from CVD. The characterisation of taxonomic composition of microbiome in the analysed tissues was performed using the method of 454 pyrosequencing of variable region IV-V of the bacterial 16S rDNA. The present taxons were determined by comparing the obtained sequences with the Human Oral Microbiome Database. The common...
|
|
|
Comparison of surface ferritin with levels of CRP and erythrocyte sedimentation rate as markers of acute-phase inflammation.
STRUČKOVÁ, Daniela
The aim of the study is to compare the levels of ferritin, CRP and the rate of erythrocyte sedimentation. Ferritin and CRP belong to the acute phase proteins that are formed in increased levels in inflammatory conditions and certain diseases. CRP is one of the most important markers of acute phase inflammation for high sensitivity, it is increasing hours after the onset of the acute phase reaction and rises more than a hundred times during the first days. CRP was measured on a Beckman Coulter AU 680 analyzer using the reaction of antigen-antibody and photometric measurements. The ferritin measurement on the Architect analyzer is a two-stage immunoassay of the immunological chemiluminescent microparticle using. The resulting reaction is measured in the optical system. Examination of the rate of erythrocyte sedimentation is frequent, although not specific to a particular disease. However, it can be used in diagnosing or monitoring of the effect therapy efficiency. The principle of measuring on Alifax Test analyzer is monitoring the aggregate capacity of red cells through optical density. These three parameters were measured by 1000 patients, the ratios of all measured values were calculated and compared with each other by means of linear regression graphs and the calculated correlation coefficients. The correlation coefficients have shown a low degree of dependence between ferritin and CRP and between CRP and age. The medium degree of dependence was proofed between ferritin and ESR parameters, between CRP and ESR and between age and ferritin, and age and ESR. All samplies were performed lege artis according to the instructions of the standard operating procedure of the company synlab czech, s.r.o. After collecting, these samples were measured in the laboratory synlab czech, s.r.o., Vrbenská 197/23, České Budějovice.
|
|
|
Laboratory methods using in diagnostic morbus Bechterew
FAROVÁ, Veronika
The aim of my bachelor thesis was to get familiar with laboratory methods, which are used in the diagnostics of morbus Bechterew. Very important for the diagnostics of morbus Bechterew is the marker HLA-B27, which is identified by screening, with the help of flow cytometer and then is the presence confirmed or refuted with the help of molecular-biological methods. Other laboratory examination, which is essential when it comes to interception and observation of the activities of morbus Bechterew, is the determination of the C-reactive protein level, which is an indicator of inflammatory reaction in the body of the patient. The last examination I got familiar with was the determination of the sedimentation speed of erythrocytes, which is used as an indicator of ongoing inflammation in the body. In the theoretical part of the thesis I focused primarily on the definition of morbus Bechterew. I dealt with the question what are the symptoms of the disease, then also according to which criteria is the disease diagnosed and what treatment is applied. In the second part of the theoretical part I focused primarily on the major histocompatibility complex, which is highly connected with morbus Bechterew. I dealt mostly with the structure and presentation of antigens by the means of MHC molecules of class I. I also mentioned the structure and the function of C-reactive protein. In the practical part I described laboratory methods used in diagnostics of morbus Bechterew. The first method I tried was screening examination of HLA-B27 marker by the means of flow cytometry. I introduced how the following processes should look like: collection of biological material, preparation of the sample and the analysis made on the flow cytometer. At the end of this part I put an example of the analysis results, how do the results look like when they are positive, negative, or in the grey zone. As a second method I introduced genetic typing of HLA-B27 molecule, which is made by the means of real time PCR. I mentioned the preanalytical part and the process of preparation. Moreover I dealt with the measuring of C-reactive protein by the means of nephelometry and then I also mentioned examination with the help of a test strip. The last method I tried was measuring the sedimentation speed of erythrocytes, I mentioned the principle of method, the process of measuring and the measured results as well. In the last part of my bachelor thesis I worked with the data I got from Motol University Hospital, more precisely from the Department of Immunology, 2nd Faculty of Medicine of Medicine, Charles University in Prague and Motol University Hospital. During the year 2017, overall 452 patients were tested on the presence of HLA-B27 marker and 75 of them tested positive. Then I worked only with HLA-B27 positive patients. I researched how much is the disease represented in both sexes, what age do the HLA-B27 positive patients have and what is their diagnosis.
|
|
|
Detection of Farthest Node in Wireless Sensor Networks
Pfudl, Tomáš ; Morávek, Patrik (referee) ; Šimek, Milan (advisor)
Master’s thesis in the first part focuses on the effectiveness of communication in the selection of reference nodes during the anchor-free localization in WSN. In this part two anchor-free localization algorithms AFL and CRP are explored. Knowledge learned on the selection of reference nodes and communication complexities of these algorithms are used in the second part. The second part is aimed at eliminating weaknesses in communication and subsequently proposed a new algorithm for selection of references. The principle of the newly proposed algorithm CASRU (Centralized Algorithm for Selection of Reference Nodes) is based on the principle of CRP. CASRU algorithm builds logical tree structure and then filters the communication through it. New algorithm achieves much greater success in the selection in randomly distributed networks while reducing the amount of communication load.
|
guest ::
